Analysis Note

ControlA431 cell lysate

Application

Research CategoryNeuroscience

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Numb in HeLa and A431 cells.

Detect Numb using this Anti-Numb Antibody, clone 6G10.1 validated for use in WB & IC.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Numb plays a key role in neurogenesis and in cell fate decisions. It maintains self-renewal properties in neural progenitor cells, which are also called radial glial cells (RGCs). It functions through inhibition of Notch signaling. Additionally, it is suggested to mediate local repair in brain ventricular wall damage.

Immunogen

KLH-conjugated linear peptide corresponding to human Numb isoforms 1 and 2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Numb in 10 µg of A431 cell lysate.

Specificity

This antibody recognizes Numb isoforms 1 and 2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~70 kDa observed. Uniprot describes four isoforms at ~71 kDa (isoform 1), ~66 kDa (isoform 2), ~69 kDa (isoform 3), and ~65 kDa (isoform 4).